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| Spilki,Fernando Rosado; Franco,Ana Cláudia; Roehe,Paulo Michel. |
| Specific IgM, IgA, IgG1, IgG2, as well as neutralizing antibody responses were evaluated in sera of calves experimentally infected with two isolates of bovine herpesvirus type 1 (BoHV1) of distinct subtypes (subtype 1, BoHV1.1; subtype 2a, BoHV-1.2a). No significant differences were observed in the antibody responses induced by each BoHV-1 subtype. The antibody responses following primary acute infection were characterized by an increase in specific IgM and IgA levels between days 2 and 14 post inoculation (pi). IgG1 was detected from days 11 to 30 pi. IgG2 was detected on the sample taken on day 30 pi. Reactivation of infection following dexamethasone administration induced a significant rise in IgA levels, whereas IgG1 and IgG2 levels, which were at high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine herpesvirus type 1; Infectious bovine rhinotracheitis; Immunoglobulin subclasses; ELISA. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200021 |
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| Franco,Ana Cláudia; Hübner,Sílvia de Oliveira; Oliveira,Anna Paula de; Batista,Helena B. de C. Ruthner; Roehe,Paulo Michel; Rijsewijk,Franciscus A.M.. |
| Bovine herpesvirus 5 (BoHV-5) is a important cause of viral encephalitis in cattle in South America. Within the framework of developing a differential vaccine against BoHV-5, a deletion mutant was constructed based on a Brazilian BoHV-5 isolate. The target of the deletions were genes that code proteins implicated in the neurovirulence of BoHV-5, the glycoprotein I (gI), glycoprotein E (gE) and membrane protein US9. To construct the deletion mutant of BoHV-5, the flanking regions of all three genes were cloned in a prokaryotic plasmid. This deletion fragment was co-transfected with the viral DNA into bovine cells. Identification of deletion mutants was performed by immunostaining with an anti-gE monoclonal antibody. One of the gE negative viral populations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine herpesvirus 5; BoHV-5; Deletion mutant; Glycoprotein genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400016 |
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| Lima,Francisco Esmaile de Sales; Batista,Helena Beatriz Ruthner; Campos,Fabrício Souza; Kunert Filho,Hiran Castagnino; Cibulski,Samuel; Finoketti,Fernando; Arantes,Thalita; Spilki,Fernando; Roehe,Paulo Michel; Rijsewijk,Franciscus Antonius Maria; Franco,Ana Cláudia. |
| This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-5; V mRNA; Transfection. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007 |
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